Part:BBa_K1763439
MaSp1/2[2:1]-12(T7)
This composite part consists of the MaSp1/2[2:1]-12 construct (BBa_K1763438) sublconed with a T7 RNA polymerase promoter and a strong RBS (BBa_K525998). This construct is intended to generate the MaSp1/2[2:1]-12 construct in high quantities in E. coli with an inducible T7 RNA polymerase system.
Biology
The T7 RNA polymerase expression system has been developed for large scale protein expression in E. coli. Strains carry an inducible T7 RNA polymerase gene incorporated into their genome. This allows very specific induction of protein expression because the T7 RNA polymerase promoter is not found in E. coli.
A common laboratory strain for protein expression is BL21(DE3), which is what the 2015 UCLA iGEM team used in this project. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1302
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage
This construct should be transformed into an E. coli strain that can produce T7 RNA polymerase under induction such BL21(DE3).
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